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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
Rabbit Igg Polyclonal Lrrc8d Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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affinity purified, polyclonal rabbit igg to gp2 antibody  (GA Generic Assays GmbH)
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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rabbit polyclonal igg anti s1 rbd protein  (Sino Biological)
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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il-7 affinity-purified anti-human il-7 rabbit polyclonal igg antibody  (PeproTech)
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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protein g-affinity-purified anti-spike (sars-cov-2) rabbit polyclonal igg antibody catalog number pab31705  (Abnova)
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( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and <t>LRRC8D.</t>
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Image Search Results


( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and LRRC8D.

Journal: Science Advances

Article Title: Regulation of volume-regulated anion channels alters sensitivity to platinum chemotherapy

doi: 10.1126/sciadv.adr9364

Figure Lengend Snippet: ( A ) The heatmap represents LC-MS/MS analysis of NPEPPS FLAG pull-down using protein lysates from BCa cell lines (KU1919 and T24; n = 3 independent lysates per cell line). The relative strength of interaction is scaled from 0 to 1. Genes annotated as playing a role in platinum drug resistance by Huang et al. are highlighted. Results from a CRISPR screen reported in Jones et al. identifying synthetic gene-to-drug interactions against cisplatin-based chemotherapy in treatment-resistant cell lines are shown. *FDR < 0.05. ( B ) Proteins identified in the NPEPPS FLAG screen as black dots and the VRAC subunits (LRRC8A-E) as red dots are mapped onto the CRISPR screen results. NPEPPS is highlighted in orange. ( C ) Affinity tag (FLAG), or IgG control, IP of protein lysates from NPEPPS FLAG in KU1919 and T24 cells was immunoblotted for NPEPPS, LRRC8A, and LRRC8D.

Article Snippet: NPEPPS and LRRC8A have been probed using the rabbit polyclonal NPEPPS antibody (1:1000; Origene, TA308014), rabbit IgG polyclonal LRRC8A antibody (1:1000, LSBio, LS-C290818 and LS-B16989), and rabbit IgG polyclonal LRRC8D antibody (1:1000, Sino Biological, 104245-T32).

Techniques: Liquid Chromatography with Mass Spectroscopy, CRISPR, Control